Biloxi to Gainesville, Florida: Road Trip Guide & Distance

How far is Biloxi from Gainesville, Florida?

The distance from Biloxi to Gainesville, Florida is 395.8 miles (636.9 km) as the crow flies. Gainesville, Florida is located E of Biloxi. By car, the driving distance is approximately 474.9 miles, taking about 9h 33min. A direct flight would take roughly 1h 18min. Both are located in United States — Biloxi in Mississippi and Gainesville, Florida in Florida.

This is a solid day of driving. Be sure to take breaks every 2-3 hours to avoid driver fatigue, and plan your meals ahead of time. Heading East means you'll be driving into the sunrise if you start early. Keep your windshield clean for the best visibility.

Coordinates come from public place data for Biloxi and Gainesville, Florida. The driving distance is estimated from straight-line distance with a road-factor model, so confirm the route in your navigation app. Fuel, flight, bus, and train values are planning estimates and can change by date, provider, road closures, and border rules.